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Laser Simulated Fluorescence in Paleontology | Dr Michael Pittman | TEDxLingnanUniversity


so today I’m going to be talking about

some developments in the world of

dinosaurs and share with you some my

journeys back in time so dinosaurs are

one of the jewels in evolutions crown

animals so amazing that is almost kind

of beyond belief some of the longest

heaviest tallest animals that ever walk

the earth

giants that had razor-sharp smiles and

armored dinosaurs that wielded bone

crushing tail weapons dinosaur fossils

themselves are really a privilege to

study but to know that in their final

encore they took to the air well frankly

it’s unbelievable over the last 200

years paleontologists have traveled the

world in search of dinosaur skeletons

pretty much to most pockets of it from

the coldest depths to the hottest

infernos and this is continuing

generation after generation

so sometimes very occasionally

paleontologists they’re basically

confronted with the exceptional fossils

like this where you actually have the

preserved stomach contents fossils that

preserve soft tissues and even fossils

that preserve feathers birds are a type

of dinosaur a 200 year old theory that

theories well-supported it’s kind of

like gravity

that’s such the support that we have so

how about with having you know all the

dinosaurs around and knowing that the

stuff that’s absent and realizing that

what you had in front of you really was

there all along so having a beautiful

fossil like this and realizing that what

you had is that so what is this sorcery

how could you get something so different

well this has to do with laser

stimulated fluorescence or LSF for short

using high-powered lasers and shining

them at fossils to make the fossils glow

in the dark so what happens is that the

laser light you shine a violet laser in

a dark room and it causes the fossils to

glow specifically the soft tissues

around the bones and when that happens

you get amazing details and this

technique has been developed by my

collaborator Tom Kay of the foundation

for scientific advancement and we’ve

I’ve been traveling with Tom around the

world

scanning specimens with lasers to try

and reveal amazing things so in the last

20 years in actually our part of the

world in northeast China they’ve been

wonderful fossils that have been found

fossils such as this one

which preserve feather and us bone and

soft tissue data that has allowed us to

reconstruct the evolution of birds from

an ancestor shared with bird-like

dinosaurs like the Raptors and it’s even

allowed us to reconstruct how feathers

evolved from very primitive filament

like structures as well as these fossils

others from different dinosaurs have

been revealed to be early

representatives

different dinosaur groups are really

filling in gaps in the early history of

those groups and one of those includes

this example so this is an early

Triceratops relative called setec

asaurus and it lived a hundred and

twenty million years ago in northeast

china so this animal we will be using

with LSF to reveal the most detailed

reconstruction of color in any dinosaur

ever so the reason this worked is

because the laser causes the skin of the

fossil to fluoresce really brightly

revealing details that you can’t see

otherwise so what we were able to do is

use all this information from from the

laser and the actual muscles themselves

to create pretty much the most detailed

reconstruction of an animal a dinosaur

ever you know from its actual you know

physique to the actual skin patterns

themselves and what you’ll notice is

that the top of the animal is rather

dark and the underside is lighter so we

interpreted this as being an example 120

million year old example of

countershading so this is a very common

phenomenon in the world today whereby

animals have different colors across

their body and the way it works is in

this example here with this mammal the

sun shines down onto it and with a

darker top the sunlight lights it up

with the sun casting a shadow over the

body this lighter part then comes in

shadow so the idea is you have a kind of

canceling out effect that ends up

camouflaging the 3d shape of an animal

so if you imagine if there was a lion

somewhere in the courtyard over there it

would have a more difficult time of

spotting this animal if it did not have

if it’s a basically didn’t have this so

that’s what we think we have into tack

asaurus but the thing that was even more

interesting is

the position of this coloration so it’s

very low on the body and we scratched

our head and had that Eureka moment when

we found that this is exactly the same

thing we see in animals that live in

environments where you don’t have as

much direct sunlight diffuse light

animals that live in forests so in this

deer for example the boundary between

the dark and light is positioned low on

the body so what we were able to do is

take that you know beautiful 3d

reconstruction two experiments to

simulate we know where will that line

fall if you had a light source shining

on the model and it matched the fossil

so you know it was isn’t 120 million

years ago dinosaurs were doing these

things that we can see you know if we go

into the hills behind us to see the

local deer that we have in Hong Kong

with small species so yeah that was

pretty fantastic so the other thing

you’ll notice about this critter all

this stuff is soft tissue and it’s scaly

you know most dinosaurs are mostly

covered in scales you notice the tail

though there’s something come broom

thing going on there it’s got these

bristle like structures now from what I

was seeing before you know birds are a

type of dinosaur and the bird-like

dinosaur also had feathers so t-rex

believe it or not we have examples of

their early relatives with feathers as

well so t-rex was a feathered animals so

but these guys are all over here and

this evolutionary tree some of dinosaurs

is as such with Triceratops being a

relative of psittacosaurus so if those

peculiar structures you know what are

they are they a kind of primitive

filament like feather and that would

suggest that you know feathers evolved

right at the beginning of dinosaurs in

the first dinosaurs are these something

different is it actually something

independent of those feathers so that’s

kind of a really important question that

we wanted to use the laser to shed some

light on and so we’re able to create

images like this

which the laser is actually able to

distill out chemical information so you

notice that this is orange the original

color is has since been gone mostly gone

from the specimen so this is reflecting

a chemical signature and you’ll notice

that it’s similar to this orange here

now this is actually the boundary of the

skin and I said that most color

disappears and that’s because these

black dots are remnants of melanin the

same pigment that gives your eyes and

your hair their color so the fact that

these bristles match the unpigmented

skin suggest that they probably have a

similar origin they probably have are

related to keratin now we did lots of

comparisons with these structures we

were keen to find out what they were

like and in the bird like dinosaurs you

get these primitive filament like

structures and these structures we found

were very similar to them but they

weren’t absolutely the same what we did

is actually look at very peculiar

structures that you find in modern birds

this is a horn screaming and you can see

why it might have got that name from its

expression they have this really strange

spine on their head which is very very

very rare amongst birds and this is not

a feather everything else on its body is

apart from the scales on its feet but

this very strange structure here and we

found that those things on the attacker

sources tail were very similar to it so

we kind of were like wow okay well that

kind of a bit puzzling if this is not

relevant what is it so it kind of really

threw a spanner in the works and even

the laser revealing these new insights

what it showed is that really we need to

be finding more clues to finally resolve

this debate so you know the laser at the

moment is you know at the cutting edge

of resolving the actual physical

appearance of dinosaurs so one of the

bottlenecks that we have in paleontology

is comment to all

all sciences is actually finding

dinosaurs so I spend my summers in the

Gobi Desert in China when it’s rather

hot and spend weeks and weeks kind of

looking at the ground it’s difficult to

find dinosaur fossils it’s even more

difficult to find tiny little animals

such as little mammals so in the time of

dinosaurs the mammals were all pretty

small no bigger than a cat or a dog and

so that was one of the things so as a

dinosaur time mem ologist of Mesozoic

mammalogists you’re kind of resigned to

the fact that living through tons and

tons of material and siming it is kind

of part of the job

so that was just what you know what

people expected and you know after

you’ve sift it you spend a lot of time

under a microscope picking through and

trying to see if you might get a fossil

tooth or bone but you know

paleontologists are easily imagined t’v

and quite early on they came up with

this really neat trick and that’s that

in areas where you have ants in the

fossil at locality you can actually pick

up the debris formed around an anthill

and look there for fossils and the

reason is that ants they pick together

or some species and at least they pick

together particles of rock are about the

same size so they’re basically the snap

having a natural way of concentrating

these fossils so paleontologists prima

knowledge is for a long time would

basically basically be wrecking and

hills around the world but you know we

thought we could maybe do better and so

we kind of put our heads together and

this thought that well we can probably

use the laser fluorescent properties of

the fossils to make you know this kind

of process less cumbersome more

efficient so in this slide here you can

see that this part is fluorescing really

well under the laser and you know some

other bones don’t pharrell different

parts of the bones for us with different

properties you know the actual matrix

holding the bone it can have different

fluorescent properties so the idea is

the fact that things fluoresce

or glow with different colors we use

that to try and find fossils so we

developed this sorting machine oh and

when you show it to various colleagues

they kind of well they didn’t think but

it was kind of close to that they were

kind of we were managed to sort through

fossils that maybe take a hundred let me

perhaps even a thousand times faster

using this method so it was really kind

of a nice step forward so that’s

something that we’re we’re hoping will

help with the rate of discovery in the

field another bottleneck is actually

preparing fossils so I’ve had experience

preparing fossils myself but you really

need to be extremely patient and into a

skill to take them out and you know

preparing a fossil can actually take

years and years to do um that’s a big

problem if you’re trying to kind of find

something and want to work on it like

how ones are gonna be ready you know so

we also managed to finds ways of using

the laser to help with that and the

specific property is as follows so this

is a proportion that isn’t fluorescing

and with the laser you can see that what

the laser can do in certain types of

fossils is actually bring out the detail

much more clearly but more importantly

can actually look a few millimeters into

the rock so it’s kind of like a very

shallow x-ray so the implications for

that is that normally when you prepare

the preparator has to basically rely on

the tiny bits of bone they can see to

know when to continue and not so it’s

why it’s such a skilled job so with the

laser you have a way of kind of knowing

if you’re going to hit bone a bit kind

of earlier than you would so that should

improve the rate at which we get

prepared the fossils out for study so

really with the LSF not only is it a way

of kind of dealing with solving old

puzzles in the field you know the origin

of birds the the actual appearance and

evolution appearance and dinosaurs but

actually as a way of eliminating or

narrow widening the bottlenecks that

limit the rate of discovery in the field

so I’ve hope you enjoy my talking

and thanks very much [Applause]

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